Table 2.
Proteomic identification of nitrated proteins found in EAD IPL
| gI accessioning number | Name | Mowse score | pI | Apparent molecular weight (Mr) | # peptides matched | Protein nitration (% control) | Probability |
|---|---|---|---|---|---|---|---|
| gi|32189392 | Peroxiredoxin 2 (Prx2) | 135 | 5.67 | 21,918 | 11/34 | 187 ± 0.15 | <0.05 |
| gi|4507645 | Triose phosphate isomerase (TPI) | 74 | 6.51 | 26,807 | 6/18 | 463 ± 63.5 | <0.02 |
| gi|4885281 | Glutamate dehydrogenase (GDH) | 112 | 7.66 | 61,701 | 14/40 | 376 ± 29.1 | <0.05 |
| gi|913159 | Neuropolypeptide h3 | 104 | 7.42 | 21,027 | 9/36 | 23.5 ± 0.04* | <0.02 |
| gi|4505753 | Phosphoglycerate mutase 1 (PGM) | 91 | 6.75 | 28,769 | 9/32 | 230 ± 4.20 | <0.05 |
| gi|4502317 | H+ transporting ATPase | 83 | 7.71 | 26,186 | 7/13 | 84.8 ± 0.05* | <0.04 |
| gi|4503571 | α‐Enolase | 191 | 6.99 | 47,350 | 20/48 | 516 ± 25.1 | <0.01 |
| gi|4930167 | Fructose 1,6‐bisphosphate aldolase (ALDO1) | 80 | 8.39 | 39,720 | 7/18 | 199 ± 21.5 | <0.04 |
*With an n= 4, if one blot has a particularly less intense protein spot for a statistically significant protein spot, this can cause an overall lower level of protein nitration. The overall % protein nitration is based on the average of protein nitration levels of all statistically significant protein spots. Ideally, one should have a greater n to avoid this potential problem, but samples from EAD are extremely rare, so we are fortunate just to have n= 4.