FIGURE 6. Caspase proteolysis of integrin β4 promotes apoptosis.
JEB pools stably expressing GFP vector or GFP-tagged WT-β4, D1109E-β4, or Tr-β4 were treated with 5 μg/ml TRAIL for the indicated times, and apoptotic nuclei were scored as described under “Experimental Procedures.” Data are presented as the mean ± S.E. of three experiments (left panel). *, p < 0.05 versus D1109E; ***, p < 0.001 versus D1109E. The expression of each construct in JEB pools before TRAIL treatment was determined by immunoblotting using a GFP antibody (right panel).