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. 2010 Feb 8;188(3):325–333. doi: 10.1083/jcb.200910083

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© 2010 Tsai et al.

This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.jcb.org/misc/terms.shtml). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 3.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/3.0/).

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Figure 2. — EGF induces HuR-dependent nuclear export of Grb7. (A) Immunohistochemistry of rat DRG neurons (top) and P19 cells (bottom) transfected with control or HuR siRNA. Colocalization of Grb7 and DAPI was quantified with the Pearson correlation coefficients and is shown on the right (*, P < 0.05). (B) Western blot analysis of nuclear and cytoplasmic fractions from P19 cells transfected as indicated in the presence or absence of EGF. (C) Mammalian two-hybrid assay in P19 cells transfected with Gal4-HuR and serial VP16-Grb7 deletion constructs. N-terminal deletions are named according to the number of deleted amino acid residues. (D) FISH detecting endogenous KOR mRNA in DRG neurons (top) and P19 cells (bottom) transfected with WT CFP-Grb7 or Δ9 CFP-Grb7. Colocalization of KOR mRNA and DAPI was quantified with the Pearson correlation coefficients and is shown on the right (*, P < 0.05). (A and D) White dotted lines outline the cytoplasm of the individual cells. (A, C, and D) Error bars represent SDs. (E) Western blots of P19 cells transfected as indicated, in the presence or absence of EGF. Bars, 25 µm.