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. 2010 Feb 8;188(3):429–441. doi: 10.1083/jcb.200906043

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© 2010 Tamada et al.

This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.jcb.org/misc/terms.shtml). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 3.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/3.0/).

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Figure 6. — The involvement of myosin V in inherent neurite turning. (A–D) Hippocampal neurons, which had been transfected with cDNAs for Venus (A), MyoVaHD-Venus (B), MyoVaHD-Venus plus MyoVa/IRES/mRFP (C), or MyoVaHD-Venus plus mRFP (D), were reaggregated and plated on 2D PDL/laminin substrates. Each panel is a composite of 30 fluorescent images acquired under the same optical conditions, which causes both saturation and stray light artifacts in the central images where the neuronal cell bodies are concentrated. In the cases of double transfection (C and D), Venus fluorescence (green) and mRFP fluorescence (magenta) have been superimposed. (E) The y axis represents the curvature of the distal 100 µm of neurites that express the indicated transgene products. Positive values represent rightward turning. ***, P < 0.001 (Bonferroni’s multiple comparison test). (F) The y axis represents the estimated length of neurites that express the indicated transgene products. (E and F) Numbers in parentheses indicate the total number of neurites examined. Data represent mean ± SEM. Bar, 200 µm.