Encapsulated NSCs express nestin (green) on the periphery and GFAP (red) in the center of cell masses after entrapment in alginate (a–b). Interestingly, some evidence of limited NG-2 expression (green) was observed in one bead, indicating oligodendrocyte progenitors (c). All nuclei were counterstained with Hoechst (blue). Images are from (a) G, 14 day (b) M-PLL, 14 day, and (c) G-PLL, 4 day, scaffolds and time points. Scale = 50 μm. Color images available online at www.liebertonline.com/ten.