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. 2009 Oct 21;29(42):13264–13273. doi: 10.1523/JNEUROSCI.1907-09.2009

Figure 7.

Figure 7.

Low-concentration CLON-DELT combination causes significant CGRP release in the spinal cord slice preparation in the absence of K+ stimulation. The ability of CLON, DELT, and the CLON-DELT combination to cause release of CGRP in spinal cord slices was investigated in the absence of K+ depolarization. CLON did not significantly increase CGRP levels above baseline levels [46.3 ± 5.9 pg/ml (basal level) vs 37.9 ± 8.5 pg/ml (CLON, 10 nm)]. In contrast, both DELT [46.3 ± 5.9 pg/ml (basal level) vs 79.2 ± 14.3 pg/ml (DELT, 10 nm)] and a 100-fold lower concentration of the CLON-DELT combination [46.3 ± 5.9 pg/ml (basal level) vs 128.5 ± 35.9 pg/ml (CLON-DELT, 0.1 nm)] were able to stimulate significant CGRP release without K+ stimulation, suggesting that the CLON-DELT combination may act synergistically at DOPs and α2ARs to externalize large dense-core vesicles. Error bars represent mean ± SEM (n = 3–11 samples/group). *p < 0.05 compared with HEPES; Student's t test.