Table 1.
Results of thermal melt assays on 61 Plasmodium proteins. Proteins are from P. falciparum unless otherwise noted. The second column refers to identifiers assigned by PlasmoDB.org.28 The third colum uses the abbreviation of "SGC" to refer to the Structural Genomics Consortium of the University of Toronto and "UW" to refer to the University of Washington. The fourth column indicates the mean Tm, the mean intra-plate standard deviation (based on 3 wells per plate), and the number of plates run for each protein. Tm's and standard deviations are in °C. "NA" means Not Available (i.e., Tm could not be estimated from the melting curves).
| Protein | PlasmoDB ID | Source | Tm in standard buffer |
|---|---|---|---|
| 2C-methyl-D-erythritol 2,4- cyclodiphosphate synthase (P. vivax) |
PVX_003920 | SGC | 45.0; 0.2 (N=6) |
| 6-phosphogluconolactonase | PF14_0511 | UW | 50.6; 0.1 (N=4) |
| 6-pyruvoyltetrahydropterin synthase |
PFF1360w | UW | 58.2; 1.0 (N=6) |
| 6-pyruvoyltetrahydropterin synthase (P. vivax) |
PVX_114505 | UW | 51.0; 1.1 (N=5) |
| Adenosine deaminase | PF10_0289 | UW | 51.1; 0.6 (N=4) |
| Adenosine deaminase (P. vivax) |
PVX_111245 | UW | 59.8; 0.5 (N=3) |
| Adenylosuccinate lyase (P. vivax) |
PVX_114710 | SGC | 44.9; 0.1 (N=3) |
| Adenylosuccinate synthetase | PF13_0287 | UW | 51.5; 0.1 (N=3) |
| Aspartate carbamoyltransferase (P. vivax) |
PVX_083135 | UW | 70.9; 0.0 (N=5) |
| CDK-related protein kinase 6 | MAL13P1.185 | Glasgow | 44.9; 0.4 (N=4) |
| Choline kinase | PF14_0020 | UW | 45.5; 0.1 (N=4) |
| Cyclophilin | PFE1430c | UW | 52.1; 0.2 (N=4) |
| D-ribulose-5-phosphate 3- epimerase |
PFL0960w | UW | 47.5; 0.6 (N=5) |
| Dihydroorotate dehydrogenase |
PFF0160c | Harvard | 47.9; 0.4 (N=1) |
| dUTPase | PF11_0282 | UW | 48.9; 0.3 (N=2) |
| Dynein light chain 1 | PFL0660w | SGC | 64.2; 0.2 (N=3) |
| Eukaryotic translation initiation factor 5A |
PFL0210c | UW | 53.1; 0.5 (N=4) |
| Farnesyl pyrophosphate synthase (P. vivax) |
PVX_092040 | SGC | 46.5; 0.3 (N=4) |
| Glutamate dehydrogenase, NADP-specific |
PF14_0164 | UW | 69.1; 0.1 (N=3) |
| Glyceraldehyde-3-phosphate dehydrogenase |
PF14_0598 | UW | 49.9; 0.3 (N=3) |
| Glycerol-3-phosphate dehydrogenase |
PFL0780w | UW | 53.9; 0.6 (N=5) |
| Glycogen synthase kinase 3 | PFC0525c | UW | 47.5; 0.2 (N=3) |
| Glycophorin binding protein | PF14_0010 | UW | NA (N=2) |
| Guanylate kinase (P. vivax) | PVX_099895 | UW | 47.2; 0.1 (N=5) |
| HSP90 | PF11_0188 | Harvard | NA (N=2) |
| Hypothetical protein, conserved |
MAL13P1.257 | UW | 54.2; 0.1 (N=2) |
| Hypothetical protein, conserved |
PFF0880c | UW | 44.6; 0.2 (N=3) |
| Methionine adenosyltransferase |
PFI1090w | UW | 44.7; 0.4 (N=4) |
| Methionine aminopeptidase 1 | PF10_0150 | UW | 43.3; 0.1 (N=5) |
| Methionine aminopeptidase 2 | PF14_0327 | UW | NA (N=1) |
| N-myristoyltransferase | PF14_0127 | UW | 59.3; 0.2 (N=5) |
| Nucleoside diphosphate kinase B |
PF13_0349 | UW | 61.8; 0.2 (N=3) |
| Nucleosome assembly protein 1 |
PKH_130240 | UW | 55.3; 0.1 (N=5) |
| Ornithine aminotransferase (P. yoelii) |
PY00104 | SGC | 47.0; 0.2 (N=3) |
| Orotidine 5' monophosphate decarboxylase |
PF10_0225 | UW | 65.6; 0.1 (N=3) |
| Peptidase | PF14_0517 | UW | 49.8; 0.5 (N=3) |
| Phosphatidylethanolamine- binding protein (P. vivax) |
PVX_123630 | UW | 42.3; 0.4 (N=6) |
| Phosphoethanolamine N- methyltransferase |
MAL13P1.214 | UW | 52.4; 0.6 (N=3) |
| Phosphoglycerate kinase | PFI1105w | UW | 45.1; 0.1 (N=3) |
| Phosphoglycerate mutase | PF11_0208 | UW | 57.0; 0.0 (N=3) |
| Phosphomethylpyrimidine kinase |
PFE1030c | UW | 62.6; 0.2 (N=3) |
| Protein-L-isoaspartate O- methyltransferase |
PF14_0309 | SGC | 48.8; 0.2 (N=3) |
| Rab11a GTPase | PF13_0119 | SGC | 41.5; 0.5 (N=4) |
| Rab18 GTPase | PF08_0110 | UW | 44.0; 0.2 (N=4) |
| Ribonucleotide reductase, small subunit (P. vivax) |
PVX_086155 | SGC | 46.3; 0.8 (N=3) |
| Ribose 5-phosphate isomerase |
PFE0730c | UW | 55.5; 0.4 (N=5) |
| Ribosomal RNA methyltransferase |
PF13_0052 | SGC | 38.1; 0.2 (N=4) |
| S-adenosyl-homocysteine hydrolase |
PFE1050w | UW | 43.8; 0.3 (N=4) |
| Spermidine synthase | PF11_0301 | SGC | 44.3; 0.1 (N=4) |
| Superoxide dismutase (P. berghei) |
PB000490.02.0 | UW | 58.6; 0.3 (N=5) |
| Superoxide dismutase (P. knowlesi) |
PKH_142350 | SGC | 60.4; 0.5 (N=3) |
| Thioredoxin | PF14_0545 | UW | 74.9; 0.4 (N=3) |
| Thioredoxin reductase | PFI1170c | UW | 50.2; 0.0 (N=3) |
| Tryptophan tRNA ligase | PF13_0205 | UW | 46.6; 0.1 (N=4) |
| Ubiquitin carboxyl-terminal hydrolase |
PF14_0576 | UW | 49.4; 1.1 (N=2) |
| Ubiquitin conjugating enzyme | PF10_0330 | UW | 52.0; 1.0 (N=5) |
| Ubiquitin conjugating enzyme | PFE1350c | SGC | 53.1; 0.1 (N=3) |
| Ubiquitin conjugating enzyme | PF13_0301 | UW | 55.7; 0.3 (N=2) |
| Ubiquitin conjugating enzyme | PF08_0085 | UW | 52.4; 0.2 (N=2) |
| Ubiquitin conjugating enzyme | PFC0255c | SGC | 51.0; 0.1 (N=4) |
| Uridine phosphorylase | PFE0660c | UW | 57.2; 0.1 (N=4) |