Figure 3. SAG silencing sensitizes cancer cells to radiation.

Cells after lentivirus-based siRNA silencing were seeded in six-well plates at three different cell densities in duplicates. The next day, cells were exposed to different doses of radiation followed by incubation at 37°C for 9 days for colony counting. The surviving fraction was calculated and plotted after comparison with 0 Gy corresponding controls. The sensitizing enhancement ratio (SER) was calculated as the ratio of the inactivation dose under scrambled siRNA control conditions divided by inactivation dose after SAG silencing. Shown is value ±SEM from three independent experiments.