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. 2009 Sep;11(9):2083–2094. doi: 10.1089/ars.2009.2489

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Copyright 2009, Mary Ann Liebert, Inc.

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FIG. 2. — siRNA-mediated knockdown of Grx2 in dopaminergic N27 cells. N27 cells transfected with three different primer sets (97, 206, 396) via Lipofectamine for 24 h. Control cells were transfected with scrambled siRNA (C) and mock transfected (-ve). (A) Representative RT-PCR analysis of Grx2 mRNA; actin RT-PCR was included as a control. (B) Representative dual Grx2 immunocytochemistry (Grx2) and mitotracker red staining (Mito marker) in cells transfected with 396 primer set (SiRNA) or scrambled (Control). (C) Grx2 activity after transfection with Grx2 siRNA primer sets 97, 206, and 396 versus scrambled control set in N27 cells. Activities are reported as percentage of control. (D) Representative RT-PCR analysis of Grx1 and Grx2 mRNA; GAPDH RT-PCR was included as a control. (For interpretation of the references to color in this figure legend, the reader is referred to the web version of this article at www.liebertonline.com/ars).