FIG. 4.

Effect of Grx2 siRNA mitochondrial Fe-S center-containing enzyme activities. N27 cells were treated with siRNA TS-396 for 24 h before exchange with normal RPMI media for another 48 h before measurement of corresponding enzyme activities. (A) Mitochondrial aconitase (m-ACON) and (B) complex I. *p < 0.0001 (n = 7) and *p < 0.05 (n = 4), respectively, compared with control (CONT, mock-transfected; siRNA, 396 transfected, reported as percentage of control). (C) Representative IRP binding in mock transfected (C) or 396-transfected (siRNA); densitometric quantification was performed by using β-mercaptoethanol (+bME) as a normalizing control. Experiments were repeated 3 times with n = 3.