FIG. 3.

SS-31 and SS-20 reduced MPP⁺-induced cell death in SN4741 dopamine cells. SN4741 cells were treated with MPP⁺ (100 μM) for 48 h in the absence or presence of SS peptides. Cell viability was quantified by the Almar Blue assay. (A) Concurrent treatment with SS-31 or SS-20 dose-dependently attenuated the reduction in cell viability induced by MPP⁺ *p < 0.05; **p < 0.01 compared to treatment with MPP⁺ alone. (B) SS-31 and S-S20 reduced apoptotic cell death induced by 100 μM MPP⁺. Apoptotic cells were evaluated by microscopy using the Hoechst 33324 dye and cells with fragmented or condensed nuclei were scored as apoptotic. **p < 0.01 compared to treatment with MPP⁺ alone.