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Asf1-H36AD37A impairs histone deposition by the HIR/Asf1 complex. Histone deposition was assayed as in Figure 2C. Plasmid DNA pre-relaxed with human topoisomerase I was incubated with 0.4 and 0.8 pmol histones H3 and H4 (lanes 2–3), 0.8 and 1.6 pmol Asf1 (lanes 4–5) or Asf1-H36AD37A (lanes 7–8) pre-incubated with 0.4 and 0.8 pmol histones H3/H4. CBP-TAP-purified HIR complex (15 μL) was added to reactions in lanes 10–16.
