Fig. 2.

CBD attenuates epileptiform activity induced by Mg²⁺-free aCSF. A, representative traces showing the effects of 100 μM CBD on Mg²⁺-free aCSF-induced LFP bursts in different regions of hippocampal slices. Dotted lines represent an individual LFP (as shown in B). B, effects of 1 and 100 μM CBD on a representative individual Mg²⁺-free aCSF-induced LFP burst. C, bar graphs showing the effects of acute treatment of increasing CBD concentrations on normalized burst amplitude (Ci), normalized burst duration (Cii), and normalized burst frequency in Mg²⁺-free aCSF (Ciii). Note that burst amplitudes have been adjusted for run-down and burst frequencies have been adjusted for run-up as described under Materials and Methods. Values are means ± S.E.M. for the last 10 LFP bursts in each condition. *, P ≤ 0.05; **, P ≤ 0.01; ***, P ≤ 0.001 (two-tailed Mann-Whitney U test).