CBD attenuates epileptiform activity induced by 4-AP aCSF. A, representative
traces showing effects of 100 μM CBD on 4-AP aCSF-induced LFP
bursts in different regions of a hippocampal slice. Dotted lines represent
an individual LFP (as shown in B). B, effects of 1 and 100 μM
CBD on a representative individual 4-AP aCSF-induced LFP burst. C, bar
graphs showing the effects of acute treatment of increasing CBD
concentrations on normalized burst amplitude (Ci), normalized burst duration
(Cii), and normalized burst frequency in the 4-AP aCSF (Ciii). Note that
burst amplitudes have been adjusted for run-down and burst frequencies have
been adjusted for run-up as described under Materials and
Methods. Values are means ± S.E.M. for the last 10 LFP
bursts in each condition. *, P ≤ 0.05; **,
P ≤ 0.01; ***, P
≤ 0.001 (two-tailed Mann-Whitney U test).