Fig. 7.

CBD displaces [³H]SR141716A binding with low affinity and lacks agonist effects in [³⁵S]GTPγS binding assays in cortical membranes. A, representative competition curves for the CB receptor agonist WIN55,212-2, the selective CB₁ receptor antagonist AM251, and CBD against 1 nM [3H]SR141716A (a selective CB₁ receptor antagonist) binding to cortical membranes. Points are means ± S.E.M. of triplicate points. B, agonist-binding curves for the CB receptor agonist WIN55,212-2, the selective CB₁ receptor antagonist AM251, and CBD stimulation of [³⁵S]GTPγS binding to cortical membranes. Points are means ± S.E.M. of triplicate points from three separate experiments.