Table 3.
Mutagenicity, DNA adducts, and abasic sites induced by compounds in strain TA100 in liquid-suspension assay
| Compound | Concen. (µg/ml) |
Percent survival |
Rev/106 survivors |
Fold increasea |
Stable adducts/ 108 nucleotides ± S.Eb |
Abasic sites/ 105 nucleotides ± S.Ec |
|---|---|---|---|---|---|---|
| DMSO (0.025 ml/ml) | 100 | 0.8 | 1.0 | 0.024 ± 0.000 | 5.9 ± 0.33 | |
| B[c]P | 20 | 71 | 3.1 | 3.9 | 1.600 ± 0.040 | 3.3 ± 0.12 |
| B[c]P 3,4-diol | 2 | 56 | 10.7 | 10.7 | 5.900 ± 0.230 | 4.2 ± 0.16 |
| 5 | 20 | 18.7 | 23.4 | 4.500 ± 0.034 | 5.3 ± 0.27 | |
| P[3,4-b]T | 10 | 24 | 4.3 | 5.3 | 0.090 ± 0.007 | 6.2 ± 0.26 |
| P[3,4-b]T 8,9-diol | 2 | 91 | 2.0 | 2.5 | 0.170 ± 0.045 | 2.9 ± 0.23 |
| 5 | 63 | 2.2 | 2.8 | 0.750 ± 0.045 | 4.7 ± 0.25 | |
| P[4,3-b]T 8,9-diol | 5 | 47 | 4.9 | 6.1 | 0.650 ± 0.045 | 5.5 ± 0.16 |
| P[3,4-b]T sulfone | 10 | 56 | 14.1 | 17.6 | Not detected | 18.4 ± 0.64 |
| 20 | 37 | 49.1 | 62.1 | Not detected | 15.1 ± 0.43 |
a
Fold increase in mutant frequency relative to that of DMSO-treated cells.
b
Average and standard error are based on two subsamples from a single biological sample for each compound. These standard errors represent technical measurement error only and should not be used to assess differences among compounds.
c
Average and standard error are based on triplicate measurements on each of three subsamples from a single biological sample for each compound. These standard errors represent technical measurement error only and should not be used to assess differences among compounds.