Table 2. Genetic characterization of batches of midges of the Culicoides pulicaris group, Germany, April 2007–May 2008*.
| Batch no. | rRT-PCR, Ct | C. pulicaris s.s. | C. punctatus | C. impunctatus | C. grisescens | C. newsteadi |
|---|---|---|---|---|---|---|
| 263/49 A | 28.57 | – | ++ | + | + | – |
| 263/49 B | 29.46 | +++ | +++ | ++ | + | + |
| 276/46B | 31.37 | – | +++ | – | + | +++ |
| 276/62A | 34.43 | +++ | +++ | – | – | – |
| 292/4A | 33.97 | +++ | +++ | – | – | – |
| 304/63A | 32.90 | +++ | +++ | + | – | ++ |
*rRT-PCR, real-time reverse transcription–PCR; Ct, cycle threshold; –, no visible band; +, faint; ++, distinct; +++, strong band after agarose gel electrophoresis of PCR products and staining with ethidium bromide.