Skip to main content
. 2009 Dec 16;29(50):15613–15620. doi: 10.1523/JNEUROSCI.3314-09.2009

Figure 2.

Figure 2.

NR1-1a binds importin α via its NLS. A, The cytoplasmic tails of NR1-1a and NR1-2a were fused to GST. B, Amino acid sequence of the C1 cassette, with the NLS in bold; asterisks denote phosphorylation sites. C, Importin α binds GST NR1-1a but not NR1-2a or GST alone in adult rat forebrain lysates. Two input lanes show 10% and 1% of input used for pull-downs. The concentration of input lysate was 0.1× or 1× (2.3 mg), as indicated. Amido black shows amount of GST fusion proteins, which have distinct molecular weights. D, The interaction between importin α and GST-NR1-1a is inhibited by preincubation with 100-fold molar excess NLS but not mutant NLS (mNLS) peptide. Ctrl, No-peptide control. The presence of two bands is due to the fact that the importin α antibody recognizes importin α1 [molecular weight (MW) 61 kDa] as well as importin α2 (MW 58 kDa).