FIG. 4.

Time-course and dose responsiveness of transcription of selected genes. Mouse lung fibroblasts were grown and treated as described in “Materials and Methods” section, with a range of concentrations of ITE or TCDD or with DMSO vehicle for 4 h (A), or were treated with DMSO, 0.5μM ITE, or 0.5nM TCDD for 2–24 h (B). Total RNA extraction and RT-qPCR were performed as described. Expression levels shown are relative to DMSO and are normalized to Gapdh assayed concurrently in the same samples. Representative of at least two experiments.