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. Author manuscript; available in PMC: 2011 Jan 29.
Published in final edited form as: Chem Biol. 2010 Jan 29;17(1):57. doi: 10.1016/j.chembiol.2009.12.009

Figure 2.

Figure 2

FrbH reaction. (a) Proposed order of reaction where CMP attachment occurs before decarboxylation. (b) HPLC analysis of FrbH reaction product. (c) MS/MS data of CMP-5'-3APn. (d) HPLC analysis of FrbH-K466A mutant, which should remove decarboxylase activity. (e) HPLC analysis of FrbH-K38A mutant, which should remove nucleotide transferase activity.