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. Author manuscript; available in PMC: 2011 Jan 29.
Published in final edited form as: Chem Biol. 2010 Jan 29;17(1):65. doi: 10.1016/j.chembiol.2009.12.010

Figure 1. SAHA alters the biochemical properties of the Sin3 complex.

Figure 1

HDAC assays were performed on 3H acetylated core histones with a. FL-BRMS1 purified complexes (top panel) or b. FL-ING2 purified complexes (top panel). DMSO and SAHA labels indicate that complexes were purified from 293T cells treated for nine hours with these compounds. The SAHA (in vitro) label indicates that complexes were purified from untreated cells and SAHA was added directly to the deacetylation reaction. Amounts of complex used in the assays were normalized to levels of FL-BRMS1 (a) (lower panel), or to levels of FL-ING2 (b) (lower panel). c. Western blot analysis of FLAG tagged proteins bound to histone peptides.