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. Author manuscript; available in PMC: 2011 Jan 29.
Published in final edited form as: Chem Biol. 2010 Jan 29;17(1):65. doi: 10.1016/j.chembiol.2009.12.010

Figure 3. ING2 dissociates through a direct mechanism.

Figure 3

a. HDAC inhibitors or control compounds were added to whole cell extracts from FLAG-BRMS1 expressing 293T cells, purified with FLAG affinity beads and then probed by Western blot for the indicated proteins. b. quantification of (a), c. FL-BRMS1 purified complex was immobilized on FLAG beads and treated with TSA or control (ethanol) and then probed by Western blot for indicated proteins. d. quantification of (c), Bars in b and d represent the average of three experiments expressed as percent band intensity of ING2 in SAHA or TSA treated extract or complex compared to the intensity in the control treatment, normalized to intensity of Sin3a. Error bars represent +/− 1 standard deviation.