Skip to main content
. Author manuscript; available in PMC: 2011 Feb 3.
Published in final edited form as: Cell Metab. 2010 Feb 3;11(2):136. doi: 10.1016/j.cmet.2009.12.009

Figure 4. Osteoclast Progenitor Number and Mature Osteoclast Apoptosis are Increased Following FoxOs Deletion.

Figure 4

(A-D) Cells were obtained from the mice described in figure 2.

(A) mRNA levels of FoxOs by qRT-PCR and protein levels of FoxOs by Western Blot in mature osteoclasts generated from non-adherent bone marrow cells cultured with M-CSF and RANKL for 5 d; ND=not detected.

(B) Caspase 3 activity and (C) mRNA levels of the indicated genes in cells described in A. CtR=calcitonin receptor; CtsK=cathepsin K.

(D) Number of TRAP positive cells generated from bone marrow cells cultured with M-CSF and RANKL for 6 d. TRAP=tartrate-resistant acid phosphatase.

*p<0.05 by Student's t-test.