Table 1.
5' upstream | Exon 1 | 3' downstream | ||||||||||||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
PGRP | Population | sequence | UTR | Translated region | Intron | Exon 2 | sequence | Total | Neutrality | |||||||||||||||||||
L | S | π | L | S | π | L | S | π | L | S | π | L | S | π | L | S | π | L | S | π | πa/πs | Tajima's | Fu & Li | |||||
s | n | s | n | D | D' | F | ||||||||||||||||||||||
Ag_Tz | 5 | 0.002 | - | - | 0 | 1 | 0.000 | 1 | 0.003 | 1 | 0 | 0.001 | 0 | 0.000 | 8 | 0.001 | 0.338 | -1.03* | -2.32* | -2.51* | ||||||||
Ag_Mz | 1 | 0.000 | - | - | 0 | 0 | 0.000 | 0 | 0.000 | 0 | 0 | 0.000 | 0 | 0.000 | 1 | 0.000 | - | -1.11 n/s | -1.81 n/s | -1.86 n/s | ||||||||
S1 | Aa Tz | 534 | 1 | 0.000 | - | - | - | 255 | 0 | 0 | 0.000 | 74 | 0 | 0.000 | 297 | 0 | 0 | 0.000 | 32 | 0 | 0.000 | 1192 | 1 | 0.000 | - | -1.16 n/s | -1.45 n/s | -1.57 n/s |
Aa Mz | 31 | 0.013 | - | - | 3 | 0 | 0.003 | 1 | 0.004 | 4 | 1 | 0.004 | 3 | 0.023 | 43 | 0.008 | - | -1.04 n/s | 1.19 n/s | 0.65 n/s | ||||||||
Ag_Tz | 8 | 0.039 | 19 | 0.021 | 14 | 3 | 0.014 | - | - | - | - | - | 9 | 0.027 | 54 | 0.020 | 0.073 | 2.50* | 1.19 n/s | 1.82*** | ||||||||
Ag_Mz | 10 | 0.041 | 27 | 0.022 | 18 | 3 | 0.014 | - | - | - | - | - | 17 | 0.038 | 75 | 0.022 | 0.049 | 0.34 n/s | 0.62 n/s | 0.62 n/s | ||||||||
S2 | Aa Tz | 107 | 1 | 0.002 | 409 | 5 | 0.005 | 567 | 11 | 1 | 0.007 | - | - | - | - | - | - | - | 172 | 14 | 0.024 | 1255 | 32 | 0.009 | 0.030 | -0.34 n/s | -0.39 n/s | -0.43 n/s |
Aa Mz | 5 | 0.023 | 7 | 0.007 | 9 | 2 | 0.007 | - | - | - | - | - | 6 | 0.015 | 29 | 0.010 | 0.061 | 0.86 n/s | 0.89 n/s | 0.99/s | ||||||||
Ag_Tz | 1 | 0.001 | - | - | 10 | 1 | 0.003 | - | - | - | - | - | 0 | 0.000 | 12 | 0.002 | 0.015 | -1.53 n/s | -1.78 n/s | -1.99 n/s | ||||||||
Ag_Mz | 10 | 0.043 | - | - | 28 | 10 | 0.025 | - | - | - | - | - | 6 | 0.019 | 54 | 0.027 | 0.141 | 1.08 n/s | 1.21 n/s | 1.38 n/s | ||||||||
S3 | Aa Tz | 84 | 8 | 0.038 | - | - | - | 567 | 16 | 1 | 0.007 | - | - | - | - | - | - | - | 61 | 6 | 0.028 | 712 | 31 | 0.012 | 0.038 | -0.08 n/s | 0.31 n/s | 0.22 n/s |
Aa Mz | 10 | 0.014 | - | - | 23 | 9 | 0.010 | - | - | - | - | - | 5 | 0.019 | 47 | 0.011 | 0.042 | -1.66 n/s | -1.97 n/s | -2.19 n/s |
*, **, ***represent P < 0.05, P < 0.01, and P < 0.001, n/s not significant. Ag_Tz, An. gambiae from Tanzania; Ag_Mz, An. gambiae from Mozambique; Aa_Tz, An. arabiensis from Tanzania; Aa_Mz, An. arabiensis from Mozambique; L, length; S, segregating site n - total number of nonsynonymous changes; s - total number of synonymous changes; π, nucleotide diversity.