Table 1.
Differentially regulated proteins in A. fumigatus after a temperature shift from 30°C to 48°C.
| Protein | T = 30 | T = 60 | T = 120 | T = 180 | Osc |
|---|---|---|---|---|---|
| Up regulated | |||||
| Chaperone | |||||
| AFUA_1G07440 (molecular chaperone Hsp70) | 1.95 | 2.45 | 2.06 | 2.45 | No |
| AFUA_1G11180 (heat shock protein/chaperonin HSP78) | 1.18 | 1.24 | 0.77 | 1.33 | No |
| AFUA_1G12610 (HSP 70 chaperone HSP88) | 1.19 | 1.06 | 0.59 | 1.34 | Yes |
| AFUA_2G04620 (Hsp70 chaperone BiP/Kar2) | 0.22 | 0.84 | 1.50 | 1.68 | No |
| AFUA_2G09290 (antigenic mitochondrial protein HSP60) | 0.43 | 1.28 | 2.17 | 1.88 | No |
| AFUA_2G09960 (mitochondrial Hsp70 chaperone (Ssc70)) | 0.18 | 0.81 | 1.47 | 1.23 | No |
| AFUA_3G14540 (heat shock protein Hsp30/Hsp42) | -0.24 | 1.23 | 2.37 | 2.35 | No |
| AFUA_4G10010 (Hsp90 co-chaperone Cdc37) | 1.13 | 1.22 | 1.84 | 1.97 | No |
| AFUA_4G12850 (calnexin) | 0.19 | 0.59 | 1.84 | 1.58 | No |
| AFUA_5G04170 (molecular chaperone and allergen Mod-E/Hsp90/Hsp1) | 3.11 | 3.68 | 3.13 | 3.76 | No |
| AFUA_5G07340 (DnaJ domain protein Psi) | 0.50 | 1.01 | 2.31 | 1.58 | No |
| AFUA_7G01860 (heat shock protein (Sti1)) | 0.38 | 1.30 | 1.92 | 1.70 | No |
| Cell wall and cytoskeleton | |||||
| AFUA_1G02550 (tubulin alpha-1 subunit) | 1.04 | 0.92 | -0.19 | 0.48 | Yes |
| AFUA_2G07420 (actin-bundling protein Sac6) | 1.48 | 1.27 | 0.30 | 1.16 | Yes |
| Transport | |||||
| AFUA_6G07120 (nuclear movement protein NudC) | -0.64 | 0.60 | 1.79 | 1.18 | Yes |
| Energy generation | |||||
| AFUA_2G13240 (V-type ATPase, B subunit) | 0.95 | 0.66 | 0.56 | 1.02 | No |
| Defence against oxidative and nitrosative stress | |||||
| AFUA_4G03410 (flavohemoprotein) | 0.73 | 0.90 | 0.97 | 1.04 | No |
| AFUA_4G09110 (cytochrome c peroxidase Ccp1) | 0.00 | 1.29 | 1.92 | 2.03 | No |
| AFUA_5G09910 (nitroreductase family protein) | -1.18 | -0.53 | 1.02 | 1.32 | No |
| AFUA_6G02280 (allergen Asp F3) | -0.54 | 0.35 | 1.62 | 1.13 | Yes |
| Signal transduction | |||||
| AFUA_1G05610 (protein phosphatase 2a 65 kd regulatory subunit) | 1.47 | 1.47 | 0.98 | 1.27 | No |
| AFUA_4G12450 (conserved lysine-rich protein) | -0.01 | 0.21 | 1.34 | 1.09 | No |
| Carbohydrate metabolism | |||||
| AFUA_1G10350 (phosphoglycerate kinase PgkA) | 0.12 | 0.42 | 1.08 | 0.80 | No |
| AFUA_1G13500 (transketolase TktA) | 1.07 | 0.54 | -0.62 | -0.01 | Yes |
| AFUA_2G05910 (hexokinase Kxk) | 1.39 | 1.27 | 0.35 | 0.73 | Yes |
| AFUA_2G10660 (mannitol-1-phosphate dehydrogenase) | 1.70 | 1.67 | 1.09 | 1.46 | No |
| AFUA_3G08660 (isocitrate dehydrogenase Idp1) | 1.19 | 0.95 | 0.08 | 0.42 | Yes |
| AFUA_4G04680 (FGGY-family carbohydrate kinase) | 1.12 | 0.88 | 0.29 | 0.79 | Yes |
| AFUA_4G11080 (acetyl-coenzyme A synthetase FacA) | 1.64 | 1.27 | -0.10 | 0.88 | Yes |
| AFUA_4G11650 (alpha-ketoglutarate dehydrogenase complex subunit Kgd1) | 1.72 | 1.39 | 0.49 | 0.95 | Yes |
| AFUA_6G04210 (mannosyl-oligosaccharide glucosidase) | 1.29 | 1.21 | 0.77 | 0.97 | No |
| AFUA_6G04920 (NAD-dependent formate dehydrogenase AciA/Fdh) | 1.17 | 0.66 | -0.67 | 0.05 | Yes |
| AFUA_6G08050 (6-phosphogluconate dehydrogenase Gnd1) | 2.21 | 1.85 | 0.55 | 1.03 | Yes |
| AFUA_6G10650 (ATP citrate lyase, subunit 1) | 1.38 | 0.92 | -0.16 | 0.33 | Yes |
| Nitrogen metabolism | |||||
| AFUA_1G10130 (adenosylhomocysteinase) | 1.88 | 1.46 | 0.05 | 0.60 | Yes |
| AFUA_1G12840 (nitrite reductase NiiA) | 2.75 | 2.30 | 1.19 | 1.54 | Yes |
| AFUA_2G11260 (3-isopropylmalate dehydratase) | 1.18 | 0.90 | -0.09 | 0.44 | Yes |
| AFUA_4G07360 (cobalamin-independent methionine synthase MetH/D) | 1.53 | 0.66 | -1.08 | -0.08 | Yes |
| AFUA_4G07710 (pyruvate carboxylase) | 2.29 | 1.84 | 0.78 | 1.25 | Yes |
| AFUA_4G10460 (homocitrate synthase) | 1.56 | 1.04 | 0.31 | 0.88 | Yes |
| AFUA_4G13120 (glutamine synthetase) | 0.67 | 1.48 | 1.72 | 1.84 | No |
| Ubiquitination and proteolysis | |||||
| AFUA_4G09030 (aminopeptidase) | 1.58 | 1.31 | 0.51 | 1.30 | Yes |
| AFUA_5G04330 (aminopeptidase) | 1.07 | 1.08 | 0.07 | 0.35 | Yes |
| Protein biosynthesis/Translation | |||||
| AFUA_1G02030 (eukaryotic translation initiation factor 3 subunit EifCb) | 1.33 | 0.64 | -0.10 | 0.43 | Yes |
| AFUA_1G06390 (translation elongation factor EF-1 alpha subunit) | 2.00 | 1.28 | -0.51 | 0.69 | Yes |
| AFUA_2G13530 (translation elongation factor EF-2 subunit) | 1.88 | 1.48 | -0.43 | 0.62 | Yes |
| AFUA_3G08160 (eukaryotic translation initiation factor 4) | 1.81 | 1.48 | 0.84 | 1.18 | Yes |
| AFUA_4G12920 (histidyl-tRNA synthetase, mitochondrial precursor) | 1.10 | 1.20 | 0.64 | 1.18 | Yes |
| AFUA_5G05920 (glycyl-tRNA synthetase) | 1.56 | 1.26 | 0.23 | 0.83 | Yes |
| AFUA_6G04570 (translation elongation factor eEF-1 subunit gamma) | 1.20 | 1.10 | 0.22 | 0.71 | Yes |
| AFUA_7G05660 (translation elongation factor eEF-3) | 2.02 | 1.43 | -0.19 | 0.70 | Yes |
| AFUA_8G03880 (alanyl-tRNA synthetase) | 1.37 | 0.80 | -0.22 | 0.32 | Yes |
| Transcription | |||||
| AFUA_1G15620 (DEAD box RNA helicase HelA) | 1.43 | 1.40 | 0.24 | 0.70 | Yes |
| Cell cycle | |||||
| AFUA_2G17110 (cell division control protein Cdc48) | 1.01 | 1.11 | 0.50 | 0.94 | Yes |
| Down regulated | |||||
| Chaperone | |||||
| AFUA_2G13040 (mitochondrial co-chaperone GrpE) | -1.12 | -0.73 | 0.21 | -0.57 | Yes |
| Transport | |||||
| AFUA_5G03690 (CRAL/TRIO domain protein) | -1.29 | -0.61 | 0.37 | -0.53 | Yes |
| Energy generation | |||||
| AFUA_2G13010 (cytochrome c oxidase polypeptide vib) | -1.79 | -1.54 | -0.12 | -1.12 | Yes |
| Carbohydrate metabolism | |||||
| AFUA_1G09930 (glycerol dehydrogenase Gcy1) | -1.16 | -1.44 | -1.25 | -0.98 | No |
| AFUA_3G09230 (carboxylesterase) | 0.14 | -0.69 | -1.07 | -1.07 | No |
| Nitrogen metabolism | |||||
| AFUA_3G09320 (serine hydroxymethyltransferase) | 0.06 | -0.67 | -1.01 | -1.12 | No |
| Protein biosynthesis/Translation | |||||
| AFUA_6G02750 (nascent polypeptide-associated complex (NAC) subunit) | -1.35 | -1.21 | -0.85 | -1.36 | No |
| AFUA_6G12660 (40S ribosomal protein S10b) | -1.35 | -1.21 | -0.85 | -1.36 | No |
| Transcription | |||||
| AFUA_3G08580 (glycine-rich RNA-binding protein) | -1.27 | -1.18 | -0.84 | -2.09 | No |
| AFUA_8G05300 (RNA polymerase II subunit 7) | -1.38 | -0.97 | -0.72 | -1.81 | No |
Relative protein abundance is depicted as log2-ratio. Logarithmized normalized intensities of the respective time points were subtracted by logarithmized normalized intensity of time point t = 0. Osc describes whether the protein is oscillatingly regulated.