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. 2010 Jan 19;9:9. doi: 10.1186/1476-4598-9-9

Figure 5.

Figure 5

VEGF165 induces c-MET activation through a NRP1-dependent mechanism. (a) Effects of NRP1 depletion on VEGF165-mediated c-MET phosphorylation. ARCaPM cells were transfected with NRP1 siRNA or control siRNA for 48 h, serum-starved overnight, then treated with VEGF165 (10 ng/ml) for 60 min. (b) Immunoprecipitation assay of NRP1-c-MET interaction. Serum-starved ARCaPM cells were treated with VEGF165 (10 ng/ml) for the indicated times, and immunoprecipitated with anti-NRP1 (upper), or anti-c-MET (low) antibody. (c) Co-localization of NRP1 and c-MET or p-c-MET. Serum-starved ARCaPM cells were treated with VEGF165 (10 ng/ml) or PBS for the indicated times. Immunofluorescence staining of NRP1, c-MET or p-c-MET was performed and visualized by confocal microscopy. Arrows indicate co-localization of NRP1 and c-MET or p-c-MET.