Fig. 5.

The expression of individual miRNAs spliced from the miR-15a/16-1 and miR-15b/16-2 clusters was detected using polyA tailing RT-PCR from eight different tissues of 2-month-old Sprague-Dawley rats: 1, miR-15a; 2, miR-15a*; 3, miR-15b; 4, miR-15b*; 5, miR-16; 6, miR-16*; 7, RT negative control. U6 noncoding RNA was also amplified as internal controls from different tissues. For RT negative control, the reverse transcription was run simultaneously with other samples without the reverse transcriptase. PCR was performed by detecting only miR-16 from each tissue; no PCR product was visualized from agarose gel. The template for negative control of U6 was selected from heart