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. 2010 Feb 11;5(2):e9173. doi: 10.1371/journal.pone.0009173

Figure 1. Growth kinetics of CB AC133+ cells.

Figure 1

CB AC133+ cells were cultured in the presence of 40 ng/ml of stem cell factor (SCF), 40 ng/ml of FLT3 and 10 ng/ml of thrombopoietin (TPO) for 30 days. Cell numbers were determined by Trypan blue exclusion assay at the time points indicated on the graph. During the ‘lag phase’ (day 0–8) there was no significant increase in numbers with time. Starting at day 8 the living cell population increased rapidly with time at an exponential growth in numbers, and the growth rate increasing with time. Increase in the cell numbers expressed as fold increase (cell numbers at splitting/cell numbers at day 0). Data points, means ± SD. * p<0.05.