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. 2010 Feb 11;5(2):e9173. doi: 10.1371/journal.pone.0009173

Figure 7. Effect of long term in vitro expansion on CB AC133+ cell chemotaxis in response to SDF1-α and Rantes.

Figure 7

At days 15 and 30 of primary culture, CB AC133+ cells were incubated for 4 h in the presence of 50 ng/ml of either Rantes or SDF-1α. Cell migration in response to these to chemokines was significantly higher (p<0.05) than that observed in control conditions (w/o chemoatractant; gray bars), with no difference between the cells cultured for 15 and the cells cultured for 30 days. Cells incubated in the presence of 10% FBS (black bars) were used as a positive control. Bars, means ± SD. * p<0.05.