Figure 3. Results of AU correction.

For 1000 genes, out of which 100 are upregulated (red points) and 900 are stable (blue points) the log2(fold change) is plotted against the percentage of As or Us in the 3′UTRs of genes. The top panels (A,B) show data with a linear AU bias and the bottom panels (C,D) show data with no AU bias. The left panels (A,C) show original data and the right panels (B,D) show data after AU correction. An optimal linear fit (black line) passes through the data with a correlation coefficient (R²) denoted for each panel. A dotted red line denotes the 100 genes with the highest log2(fold change) values.