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. 2010 Feb 11;5(2):e9159. doi: 10.1371/journal.pone.0009159

Figure 3. Effects of ghrelin receptor ligands on the activity of GHRH neurons.

Figure 3

A to C, typical experiments where superfusions with the agonists GHRP-6 (10 & 100 nM, A) and JMV1843 (100 nM, B) increased action potentials rates in adult male GHRH neurons; the antagonist JMV3002 (1 µM) prevented from the stimulatory effect of ghrelin (10 nM, C). D–E, summaries of the effects of GHSR agonists (D: ghrelin, GHRP-6, JMV1843, JMV2952) or antagonist (E: JMV3002) on the cumulative distributions of the spontaneous action potentials of GHRH neurons. The action potential frequencies observed at the half maximal values of the cumulated histograms were averaged according to the absence (white bars) and presence of agonists and/or antagonist (coloured bars, see Methods for details). In D&E, bars and lines are the means and the sem of the numbers of experiments indicated. Statistical differences *, p<0.05; **, p<0.01; ***, p<.005 are shown (paired student-t test).