Figure 8. MstnPP aggregates and protofibrils (PF) are cytotoxic to C2C12 mouse myoblasts.

C2C12 cytotoxicity assay using the WST-1 reagent where the difference in absorbance at 450 and 630 nm directly correlates to cell density after incubation with increasing concentrations of MstnPP soluble aggregates (SA, 5–25 µM), protofibrils (PF, 5–25 µM), 25 µM MstnPP dimer and 25 µM PF/F (fibrils). Concentrations are expressed as monomer equivalents. Cells incubated in media containing buffer (B) only (50 mM Tris-HCl pH 8.5, 150 mM NaCl for soluble aggregates and dimer; 0.005 mM HCl, pH 5.3 for protofibrils and fibrils) were used as a control. Error bars represent the standard error of the mean for triplicate samples from two independent experiments. Statistical significance was calculated using a paired Student's t-test where ** P<0.01 and * P<0.05.