Table 1. DNA sequence of oligonucleotides used for polymerase chain reaction (PCR).
| Amplified fragment | Forward primer (5′-3′) | Reverse primer (5′-3′) | PCR length (bp) | Annealing temperature °C |
|---|---|---|---|---|
| Promotor |
CATGGCTAGTACGGATGTAATT |
GGAGAAACTAACCACCATCA |
250 |
55 |
| Exon 1 |
TGTTTCCGTGACGGACTGAGTA |
CCTGAGAGTCACCTGCACATTT |
330 |
56 |
| Exon 2 |
TCCTTTGTACTGTTACCCTCTC |
CTAGTCCATGGTAGAGACACAA |
530 |
54 |
| Exon 3 |
CATTATTCTTAGTAGATTAATGTG |
AAACAGTTAGTGGCAGCTGTGG |
340 |
56 |
| Exon 4 |
GTAGGGTTGTCATGAGGATTAA |
CCTCCATGGTTCTTAAATTT |
270 |
54 |
| Exon 4b |
GAAATCTGAGATCTCAGACATC |
TGGTCTGCAATTTTATTTGCAAC |
250 |
57 |
| Exon 5 |
TCAGCTTAGGCTGTTGACATCAC |
CCAGAACTGCCACGTAATACCT |
300 |
62 |
| Exon 5b |
TCCCTAGCTTACATCTGTTCCT |
TGGCAGAAATGGCTGATTAGTG |
270 |
57 |
| Exon 6 |
GCAACAGGGAAATGATTGCCTT |
AGGTTCTATCATTACACCTTCC |
410 |
56 |
| Exon 7 |
AGGAGATATGACTTCAAGATTTTGG |
CCATCCTCCAAGCACATTAGGTT |
350 |
57 |
| Intron 7 |
AGGAGATATGACTTCAAGATTTTGG |
CATGTTTGTTAAGCTAGAACTG |
900 |
56 |
| Intron 7 |
CTGCAGTAAATCATGGCATGT |
GAGAGAGAAAGAGAGAGGACAG |
650 |
52 |
| Intron 7 |
CAGTGGATACTTAACCACCC |
GAGAACATCAAGAACTTCAG |
450 |
54 |
| Exon 8 |
CTCTTGACACATCTGTTATATT |
GCCTACATCTAGTATGTATA |
270 |
52 |
| Exon 9 |
CTCAGAGCAGCATTACAAATAGG |
AACACAATGAACAGGTCTCACTG |
250 |
57 |
| Exon 10 |
CAATGCAGTAGCCCTGTCTAGA |
GGCTAACGGTACAGCCTTCTTT |
380 |
62 |
| Exon 11 |
GATAGTTCTCGGGAGTTTGATC |
GCTCTACATATCTAGATAGCAGC |
360 |
62 |
| Exon 12 and 13 |
GTCTTATCTGAATGGATGAG |
GCAAAGCTTGGATTGCTAAAGAAG |
420 |
57 |
| Exon 14 |
CTTGCTATAATGTAGACACAGGG |
AAATCCCTATCACAGCTGGAGC |
300 |
62 |
| Exon 15 and 16 |
TAGGCAGCAGAGCAGGAATTCA |
CAGTTCAATTTAAGCTACTCTC |
400 |
56 |
| Exon 17 |
AGCACATTCGCAGACTTGGTGGTA |
GTATGGATGCCAAAGATTGCCAGC |
260 |
68 |
| Exon 18 |
CCACTTTAACCACTACATCTGG |
GAGATAACTGCTCCTAGAGATG |
300 |
62 |
| Exon 19 |
CCTCCCTTTGGTTATCTCTGAA |
GCCTATGAGCCAAGGCAACAATAAAT |
270 |
54 |
| Exon 20 |
CTTGACTGGTGCGATTTACAGG |
GAAAGATAGAGGCTGTGATGGG |
430 |
60 |
| Exon 21 |
CTGTTTGGCTTGAGCTCGTGTT |
TGAGGCTGATACCCCAGTATAC |
430 |
62 |
| Exon 22 |
GGGCCAGGAGAGAATCTCCACT |
TGGATGACTCCTTCACCACTGT |
350 |
62 |
| Exon 23 |
CTGCCTTCATATTGATATAGCAC |
ATTCCTGAGACTGGTCTAGAGC |
300 |
54 |
| Exon 24 |
TTTACCATATTCATAAGCCGGG |
TGTCAGCAAATGTACACGTGAC |
450 |
60 |
| Exon 25 |
CTTCTCAGTGTGGTTGATCAAC |
CCCCAGATGATCAAAGGACTTA |
300 |
50 |
| Exon 26 |
CTGGTTCTAGTAGTTGTATGTG |
AGCTACCACACCTGGCCAGTTT |
350 |
56 |
| Exon 27 |
GCACTTCCATTAAAGTGTATAGC |
ACAAATGGAAATGGGAAAGGTGG |
420 |
62 |
| Exon 28 | AAGGTGGTATGGTGAGACTCAT | TACAGCATAAGTGACAAGCAGG | 350 | 62 |