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. Author manuscript; available in PMC: 2010 Feb 10.
Published in final edited form as: J Biol Chem. 2007 Aug 16;282(40):29504. doi: 10.1074/jbc.M705825200

FIGURE 3. Re-localization of eGFP-myo-1e to cortical granules.

FIGURE 3

Images are from a time-lapse, confocal movie of the sub-plasma membrane region of an oocyte induced to undergo exocytosis by PMA treatment (50 nM) in the presence of Texas Red dextran. A, before exocytosis (00:00), neither eGFP-Myo1e nor dextran is apparent. After the onset of exocytosis (01:10), eGFP-Myo1e (green) surrounds dextran (red), which is contained within the large endosomes generated by cortical granule-plasma membrane fusion (arrowheads). In addition, eGFP-Myo1e is also apparent on circular compartments of the same size but which do not contain dextran (arrows). B, eGFP-Myo1e is recruited to cortical granules prior to exocytosis. Images collected as in A, but at 2-s time intervals. The eGFP-Myo1e is recruited to cortical granule membranes (arrows) several seconds before the onset of exocytosis, as indicated by dextran filling (arrowheads). Time in min/s.