FIGURE 9. Csp co-immunoprecipitates with Myo1e and Myo1e co-immunoprecipitates with Csp.

In each instance, the signal in the input lane corresponds to the immunoreactivity (for Myo1e or Csp) present in 5% of the total oocyte extract (a lipid-depleted supernatant fraction prepared from oocytes using buffer A) that was used for the immunoprecipitation (IP). The control lanes represent samples where the immunoprecipitation was done using either Myo1d antibody or no primary antibody. The IP lanes show the signal obtained when Csp immunoprecipitates were probed for Csp or when Myo1e immunoprecipitates were probed for Myo1e. The co-IP lanes show the extent of co-immunoprecipitation of either Csp (with Myo1e antibody) or Myo1e (with Csp antibody).