Figure 3.
Numerical derivatives of Ka-band echo-detected field swept spectra of WT mitoNEET, H87C mitoNEET, and ferredoxin. Black: experimental data, red: simulations. Experimental conditions: frequency 30.89 GHz, π/2 width 20 ns, τ 330 ns, π width 40 ns, repetition time 5 ms, temperature 12 K. Simulation parameters for 3 contributing species of H87C: 55% g = (2.005, 1.974, 1.916). 36% g = (1.993, 1.962, 1.895), 9% g = (1.995, 1.948, 1.864). Insets: Ka-band 3-pulse ESEEM traces. Experimental conditions: frequency 30.89 GHz, π/2 width 10 ns, τ 250 ns, initial T 70 ns, T increment 20 ns, repetition time 5 ms, temperature 12 K.