Ka-band (top) and Q-band (bottom) 2-pulse ESEEM for 15N-labeled mitoNEET across the EPR envelope, with simulations (red and blue). Ka-band conditions: frequency 30.89 GHz, fields 1100, 1110, 1120, 1130, 1140, 1145, 1150, 1155, 1160, 1165 mT, π/2 width 10 ns, π width 20 ns, initial τ 110 ns, τ increment 10 ns, repetition time 5 ms, temperature 12 K. Q-band conditions: frequency 33.904 GHz, fields 1207, 1212, 1218, 1229, 1240, 1245, 1256, 1262, 1273, 1278 mT, π/2 width 28 ns, π width 56 ns, initial τ 126 ns, τ increment 8 ns, repetition time 5 ms, temperature 12 K.