Fig. 5.

mRNA levels for inflammatory mediator genes in the knee joint tissue from normal control, CAIA and CAIA + SRL mice. Knee joints were harvested on day 7 after LPS injection. Soft tissues such as skin and muscle were removed from around each joint, and the total RNA was extracted. The mRNA levels for tumor necrosis factor-alpha (TNF-α), interleukin-1-beta (IL-1β), IL-6, IL-10 and interferon-gamma (IFN-γ) were measured by reverse transcription (RT)-PCR. The values shown were normalized with glyceraldehyde-3-phosphate dehydrogenase transcript (GAPDH). Values are the means ± SD (n = 6)