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Primer list. Primers used for construction and verification of gtxA mutants. Restriction sites are underlined.
| Primer name | Primer sequence (5′-3′) |
|---|---|
| 4240 | TATCGTCGACTATCCATCGCGGCATCAG |
| 4242 | AGCTGAATTCAAGCAAGTGCTATTGCTACCG |
| 4243 | AGCTGAATTCTTATGTCGGCGATCAAACAA |
| 4245 | TATGTCTAGAGGCGTTGGTGGATAAGAGAT |
| kanR | CGATAGATTGTCGCACCTGA |
| kanF | TATGGAACTGCCTCGGTGA |
| 39F | TGATGCAATCAAAGATAAAGTCG |
| 5734R | AATCGGCATTGGAGCTTTC |
| 2871F | AACCAAACCAATCCAAGGT |
| 3270R | ATTGCCGTCTTTGCCTACTG |
