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. Author manuscript; available in PMC: 2010 Oct 1.
Published in final edited form as: J Immunol. 2009 Sep 4;183(7):4601. doi: 10.4049/jimmunol.0901754

Figure 4. Effects of hyperoxia on oxidative stress and inflammatory gene expression in Nrf2+/+ and Nrf2−/− alveolar macrophages.

Figure 4

(A) DCF staining of BAL macrophages collected from Nrf2+/+ and Nrf2−/− mice exposed to room air (left), 48 h hyperoxia (middle), and in recovery (right). Graph represents quantification of relative number of cells stained with DCF. *P ≤ 0.05 vs. room air control of the same genotype and † P ≤ 0.05, Nrf2−/− vs. Nrf2+/+ cells. The expression levels of Gclc (B) and Il-1 and Il-6 (C) in macrophages obtained from the BAL fluid were analyzed using TaqMan real-time probes. The values represented are means with SD (n = 3). *P ≤ 0.05 vs. room air control of the same genotype; † P ≤ 0.05, Nrf2−/− vs. Nrf2+/+ cells subjected to hyperoxia. (D) ELISA measurement of Il-6 levels in the BAL fluids of Nrf2−/− vs. Nrf2+/+ mice exposed to room air, 48 h hyperoxia, and during recovery. Data are means with SD (n = 4–5). *P < 0.05 vs. room air control of the same genotype; † P < 0.05, Nrf2−/− vs. Nrf2+/+ cells.