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. Author manuscript; available in PMC: 2010 Oct 1.
Published in final edited form as: J Immunol. 2009 Sep 4;183(7):4601. doi: 10.4049/jimmunol.0901754

Figure 6. The effects of hyperoxia exposure on bactericidal activity of Nrf2+/+ and Nrf2−/− peritoneal macrophages.

Figure 6

Peritoneal macrophages isolated from Nrf2+/+ and Nrf2−/− mice and supplemented without (open bars) or with GSH (filled bars) were exposed to hyperoxia for 24 h and then incubated with P. aeruginosa at 1:100 ratio for 60 min. (A) The cfu of the remaining bacteria were determined as described in Methods and bactericidal indices were calculated. † P ≤ 0.05, Nrf2−/− vs. Nrf2+/+ cells exposed to hyperoxia. § P ≤ 0.05, Nrf2−/− vs Nrf2−/−GSH of the hyperoxia exposed experimental groups. The relative expression levels of Gclc (B) and Il-6 (C) transcripts in Nrf2+/+ and Nrf2−/− peritoneal macrophages exposed to room air (RA), hyperoxia (Hy), P. aeruginosa (PA) and combination of hyperoxia and P. aeruginosa (Hy+PA) supplemented without (open bars) and with GSH (filled bars) are shown. Each bar represents the average value with SD (n = 3). *P ≤ 0.05 vs. room air control of the same genotype; † P ≤ 0.05, Nrf2−/− vs. Nrf2+/+ cells exposed to hyperoxia.