Fig. 4.
Inhibition of IL-1β-induced PKCδ phosphorylation by GTE in RA synovial fibroblasts.RA synovial fibroblasts (2 × 105/well) were treated with GTE (2.5–20 μg/ml) for 12 h, followed by stimulation with IL-1β (10 ng/ml) for 20 min. Cells were lysed in extraction buffer containing protease inhibitors, and the total and phosphorylated (p) PKCδ (A) and JNK (B) were determined by western blotting. A representative blot for each protein is shown. Equal loading of protein was verified by re-probing the blots for β-actin. Values are mean and SEM of RA synovial fibroblasts obtained from six different donors. *P < 0.05 vs treatment with IL-1β. OD: optical density; n: number of RA synovial fibroblast donors used.