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Single Holliday junction intermediates in S. pombe meiotic recombination. DNA from meiotic mus81 mutant cells was separated by two-dimensional gel electrophoresis, and DNA in the joint molecule region was visualized with an electron microscope. The junction of the four DNA duplex segments is splayed out in a “traffic circle” due to formamide in the spreading mixture. Bar indicates 0.2 μm. From Cromie et al. (2006).
