Figure 4. Delayed loss of CD34 on expanding HPC from RA patients.
Freshly isolated CD34+ cells from 17 patients and 17 controls were driven with early hematopoietins as described in Figure 3. On days 4, 8, 11 and 18, cells were harvested and analyzed for the surface expression of CD34, CD11c, CD14, and G-CSF receptor by flow cytometry. Frequencies of cells retaining CD34 (A) or acquiring CD11c (B, left), CD14 (B, middle), or G-CSFR (B, right) are shown as box plots in RA (gray boxes) and controls (white boxes) as described in Figure 1.