Table 3.

CovR/S sequence analysis in emm1 and emm81 isolates

emm- type	Source	Date	SpyCEP Copy No.a	CovR/S	Sequence Analysis
emm1	Reference	1880	9669	CovS	1bp insert=stop at 318aa
	Blood	1932	3505	Wild type sequence
	Blood	1934	1334	Wild type sequence
	HVS	1938	56	CovS	Arg to Cys at 216aa Leu to Val at 458aa
	Blood	1999	1363	CovS	Ala to Thr at 38aa Ile to Val at 332aa
	Blood	2000	700	CovS	Ala to Thr at 38aa Ile to Val at 332aa
	Blood	2000	472	CovS	Ile to Val at 332aa
	Blood	2000	3594	CovS	Ile to Val at 332aa†
	Throat	2000	786	CovS	Ala to Thr at 38aa Ile to Val at 332aa
emm81	Bloodb	1994	23447	CovR	Ala to Val at 111aa
	Blood	2001	1152	Wild type sequence
	Blood	2001	138	Wild type sequence
	Blood	2001	131	Wild type sequence
	STSS	2003	12	Wild type sequence
	STSS	2003	5	Wild type sequence

Note. Genbank accession numbers EU726232, EU726234, EU26247-EU26249, EU730696-EU730705. Aa, amino acid; Ala, alanine; Arg, arginine; Cys, cysteine; HVS, high vaginal swab; Ile, isoleucine; Leu, leucine; PCR, polymerase chain reaction; STSS, streptococcal toxic-shock syndrome; Thr, threonine; Val, valine; WT, wild type.

^acopies per 10,000 copies of gyrA measured by real time PCR at mid log phase

^bStrain H292