Fig. 4.

RT-PCR of RNA from biofilm cultures grown in Mn-supplemented or Mndepleted media. Total RNA from biofilm bacteria was serially diluted prior to amplification with gene-specific primers (as labeled in a–h) and represented in lanes 1 (most concentrated) to 4 (least concentrated). The PCR products were separated on a 1% agarose gel. The experiments were run in triplicate to confirm reproducibility. 16S RNA was run as a control.