Fig. 5.

RT-PCR of RNA from either planktonic or biofilm cultures grown in Mn-supplemented or Mn-depleted media. Total RNA from bacteria was serially diluted prior to amplification with rgg-specific primers and represented in lanes 1 (most concentrated) to 4 (least concentrated). The PCR products were separated on a 1% agarose gel. The experiment was performed in triplicate to confirm reproducibility. 16S RNA was run as control.