TABLE 2.

Pulse-chase experiments for trapping ¹⁴CO for CODH/ACS and for ACS with 1 mm TiCit, 1 mm CoA, 50 μm CH₃-CFeSP, 1 mm CH₃-H₄folate, and 11 μm MeTr by hand mixing

The experiments in this table were done by hand mixing with both pulse and chase times set at 100 s. All of the experiments without CO chase were under an N₂ atmosphere.

Reaction time	CODH/ACS	[14CO] pulse	Chase	[14C1-acetyl-CoA]	Percentage recovery in pulse/chase	Percentage recovery expecteda
s	μm	μm		μm	%
100	50	42	0	31 ± 2
			980 μm CO	28 ± 2	90 ± 9	4
			0	29 ± 2
			50 μm Mb	25 ± 2	86 ± 9.0	0
			0	27 ± 2
			15 mm CO2	1.3 ± 1.5	4.8 ± 5.5	0
	[ACSHT]
	μm
120	24	38	0	31 ± 2
			980 μm CO	32 ± 2	100 ± 9	4
		29	0	29 ± 2
			40 μm Mb	14 ± 1.5	48 ± 6	0

^aThe expected percentage recovery is based on the isotope dilution if CO must dissociate before product can be formed. It is assumed that myoglobin can bind all of the CO