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. Author manuscript; available in PMC: 2010 Feb 11.
Published in final edited form as: Neuron. 2009 May 14;62(3):400. doi: 10.1016/j.neuron.2009.03.019

Figure 2. Palisades and Somata of Bergmann Glia Were Identified Based on Anatomical Features and Sulforhodamine 101 (SR101) Co-staining.

Figure 2

(A) The red fluorescent astrocyte marker SR101 stains Bergmann glia in the cerebellar cortex of adult mice. Localization of SR101 to Bergmann glia was verified in the Kosmos mouse line expressing EGFP under the control of an S100β promoter, an established marker of astrocytes. Left, EGFP expression in Bergmann glia of the Kosmos line. Center, SR101 staining pattern in the same mouse. Right, Image overlays showing that SR101 stains Bergmann glia somata and palisade fibers (yellow, open arrow heads) in the Purkinje cell (bottom) and molecular layers (top), respectively. Molecular layer interneurons and Purkinje cells (closed arrow heads) are labeled neither in the Kosmos line nor by SR101.

(B) SR101 co-staining was used to identify Bergmann glial palisades and somata in OGB-1-AM fluorescence images. Left, OGB-1-AM staining pattern in the molecular (top) and Purkinje cell layer (bottom). Center, SR101 staining in the same mouse. Right, Overlay images, in which Bergmann glia fibers, end foot processes and somata are indicated (open arrow heads) (See also Figures S1 and S2). Molecular layer interneurons and Purkinje cells (closed arrowheads in top and bottom rows, respectively) are stained by OGB-1-AM but not SR101.

Scale bars: 15 μm.