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. 2010 Feb 5;4:2. doi: 10.3389/neuro.08.002.2010

Figure 1.

Figure 1

Fear conditioning regulates EGR-1 expression in the MGm/PIN. (A) Schematic of the behavioral protocol. (B) Western blot analysis of EGR-1 protein in the MGm/PIN of Paired (n = 8), Tone Alone (n = 8), Imm. Shock (n = 8), Delayed Shock (n = 8), and Naïve (n = 8) groups following fear conditioning. *p < 0.05 relative to Naïve, Tone Alone, Imm. Shock, and Delayed Shock groups. Representative blots can be seen in the inset. (C) Representative 10X photomicrograph of immunolabeled EGR-1 neurons in a Paired rat. (D–E) Representative 20X photomicrographs of immunolabeled EGR-1 neurons in an Imm. Shock and Naïve rat, respectively. (F) Quantification of EGR-1 labeled cells in the MGv, MGm, and the PIN of Paired (n = 3), Imm. Shock (n = 3), and Naïve (n = 3) groups. *p < 0.05 relative to Naive and Imm. Shock groups. #p < 0.05 relative to Naïve group. (G) Quantification of EGR-1 labeled cells in the MGv, MGm, and the PIN of Paired (n = 4), Tone Alone (n = 4), and Naïve (n = 4) groups. *p < 0.05 relative to Naive and Tone Alone groups.